The Washington State Department of Transportation (WSDOT) and its partner agencies developed a research program to design new and retrofit culverts. The goal of this program is to identify culvert bed configurations, designs, and associated hydraulic conditions that allow successful movement of juvenile salmonids upstream, while safely passing water, sediment, and debris downstream. WSDOT in cooperation with the Washington Department of Fish and Wildlife (WDFW) constructed a culvert test bed at the WDFW’s Skookumchuck Hatchery in western Washington State. Battelle conducted experiments to establish protocols for future research on bed conditions, culvert shape, etc. This technical report describes various protocols, such as time of day, duration of test, and density of test fish, and provides hydraulic and biological characterizations of a baseline culvert.